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|Last Updated (September 15, 2003)|
About this Database
Malaria Full-Length cDNA project
2.Construction of Full-length cDNA Library
2-3. Construction of a Full-Length-Enriched cDNA Library
In order to overcome the difficulty described in the previous section, we developed a new method to introduce a "sequence tag" at the 5'-end, which we call the "Oligo-capping" method. This method allows us to replace the cap structure of mRNA with a synthetic oligo-nucleotide enzymatically. Each mRNA product of the "Oligo-capping" contains the "sequence tags" at both ends, i.e., polyA at the 3'-end and the cap-replacing oligo at the 5'-end. With "Oligo-capped" mRNA as a starting material, a new system is developed to selectively clone the cDNA which contains both sequence tags at the respective ends. Following the scheme shown in the figure above, a cDNA library is constructed in which the content of "full-length" cDNA is significantly enriched ("full-length-enriched" cDNA library).
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