Malaria Full-Length cDNA Project (P.falciparum)
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Last Updated (September 15, 2003)

About this Database
Malaria Full-Length cDNA project

2.Construction of Full-length cDNA Library

2-3. Construction of a Full-Length-Enriched cDNA Library

In order to overcome the difficulty described in the previous section, we developed a new method to introduce a "sequence tag" at the 5'-end, which we call the "Oligo-capping" method. This method allows us to replace the cap structure of mRNA with a synthetic oligo-nucleotide enzymatically. Each mRNA product of the "Oligo-capping" contains the "sequence tags" at both ends, i.e., polyA at the 3'-end and the cap-replacing oligo at the 5'-end. With "Oligo-capped" mRNA as a starting material, a new system is developed to selectively clone the cDNA which contains both sequence tags at the respective ends. Following the scheme shown in the figure above, a cDNA library is constructed in which the content of "full-length" cDNA is significantly enriched ("full-length-enriched" cDNA library).

<< Previous: 2-2. Towards Cloning of Full-Length cDNA

  1. Background
  2. Construction of Malaria Full-Length cDNA Library
  3. Current Status
  4. Experimental Procedures

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