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|Last Updated (September 15, 2003)|
About this Database
Malaria Full-Length cDNA project
2.Construction of Full-length cDNA Library
Schematic representation of the "Oligo-capping" method
The cap structure of the mRNA is replaced with a 5'-oligo by the "Oligo-capping" method, which consists of three enzymatic reaction steps. Bacterial alkaline phosphatase (BAP) hydrolyses the phosphate of truncated mRNA 5'-ends whose cap structures have been broken down. Tobacco acid pyrophosphatase (TAP) removes the cap structure, leaving the phosphate at the 5'-end. T4 RNA ligase, which requires a phosphate at the 5'-end as its substrate, selectively ligates the 5'-oligo to the 5'-end that originally had the cap structure.
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