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|Last Updated (August 15, 2006)|
About this Database
Malaria Full-Length cDNA project
(1Department of Parasitolog, Institute of Medical Science, The University of Tokyo 2Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo)
FULL-malaria has started as a database for a full-length cDNAs from human malaria parasite, Plasmodium falciparum. Now it contains full-length cDNAs from human malaria parasite, P. vivax and murine malaria parasites, P. yoelii and P. berghei.
Full-length cDNA libraries were produced from the intraerythrocytic stage parasites using the oligo-capping method and 5'-end-one-pass sequences of random clones were determined. These and EST sequences obtained from the database were mapped onto the genome sequences along with annotated gene structures.
The protein sequences of the annotated genes of P. falciparum were used as queries to search homologous regions of the genome sequences of the other malaria parasites using TBLASTX program. Based on these homologies, the contig sequences of P. vivax, P. yoelii and P. berghei were aligned with the genome sequences of P. falciparum and displayed in the graphical genome viewer.
Vivax malaria Full-length cDNA project
(1Department of Parasitology, Sam Ratulangi University, 2Department of Parasitology, Keio University)
Murine malaria full-length cDNA project
(1Research Institute for Biological Science, Tokyo University of Science, 2 Department of Medical Zoology, School of Veterinary Medicine, College of Bioresource Sciences, Nihon University)
We have made full-length cDNA libraries from P. yoelii 17XL (XPYw) and 17XNL (XPYs).
We have developed a method to screen DNA vaccine candidates using full-length cDNA libraries and are screening cDNA clones that were produced from the intraerythrocytic parasites of P. berghei ANKA.
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