Malaria Full-Length cDNA Project (P.falciparum)
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Last Updated (September 15, 2003)

About this Database
Malaria Full-Length cDNA project

2.Construction of Full-length cDNA Library

2-1. Drawbacks of Conventional cDNA Library

The full-length cDNA, which contains the entire sequence of the mRNA, is the ultimate goal of the cDNA cloning. Unfortunately, cDNA libraries constructed by many types of conventional methods have a high content of non-full-length cDNA clones. One of the reasons for this high content is that reverse transcriptase tends to stop during the first-strand synthesis and to fall off, leaving non-full-length cDNA. Thus, non-full-length cDNA is an inevitable result of the use of reverse transcriptase for the cDNA synthesis.

Full-Length mRNA

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  1. Background
  2. Construction of Malaria Full-Length cDNA Library
  3. Current Status
  4. Experimental Procedures

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