|Home > About this Database > 2.Construction of Full-length cDNA Library|
|Last Updated (September 15, 2003)|
About this Database
Malaria Full-Length cDNA project
2.Construction of Full-length cDNA Library
2-1. Drawbacks of Conventional cDNA Library
The full-length cDNA, which contains the entire sequence of the mRNA, is the ultimate goal of the cDNA cloning. Unfortunately, cDNA libraries constructed by many types of conventional methods have a high content of non-full-length cDNA clones. One of the reasons for this high content is that reverse transcriptase tends to stop during the first-strand synthesis and to fall off, leaving non-full-length cDNA. Thus, non-full-length cDNA is an inevitable result of the use of reverse transcriptase for the cDNA synthesis.
>> Next: 2.2 For Cloning of a Full-length cDNA......
welcome your comments and feedback about our database.
Please feel free to contact us...... firstname.lastname@example.org