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of Full-length cDNA Library |
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About this Database Malaria Full-Length cDNA project 2.Construction of Full-length cDNA Library 2-1. Drawbacks of Conventional cDNA Library The full-length cDNA, which contains the entire sequence of the mRNA, is the ultimate goal of the cDNA cloning. Unfortunately, cDNA libraries constructed by many types of conventional methods have a high content of non-full-length cDNA clones. One of the reasons for this high content is that reverse transcriptase tends to stop during the first-strand synthesis and to fall off, leaving non-full-length cDNA. Thus, non-full-length cDNA is an inevitable result of the use of reverse transcriptase for the cDNA synthesis.  >> Next: 2.2 For Cloning of a Full-length cDNA...... |
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